SOM     CCCC     CTSC
    We are leaders in CRISPR/Cas9 gene targeting. We have completed over 60 CRISPR/Cas9 projects including knockouts, reporter and point mutant knockins and targeted transgenics. more details.

     Our fees are low, our wait times are short and our success rates are high. New transgenic and targeted mice that we have created are featured in over 250 publications since 2004.

    We are supported by the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center, the Clinical and Translational Science Collaborative and by research grants to Dr. Conlon from the Cystic Fibrosis Foundation.

NameRoleOfficePhone
Weihong JiangStaffWRB 2514368-2528
David LePageStaffWRB 2514368-2528
Rachel MannManagerWRB 2514368-2528
Ron ConlonDirectorBRB 623 368-1826


ctsc
Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative.

Transgenic and Targeted Mice Made by the Core

2019
  1. Miao Q, Hill MC, Chen F, Mo Q, Ku AT, Ramos C, Sock E, Lefebvre V, Nguyen H. SOX11 and SOX4 drive the reactivation of an embryonic gene program during murine wound repair. Nat Commun. 2019 10(1):4042. PMID: 31492871

  2. Donovan LJ, Spencer WC, Kitt MM, Eastman BA, Lobur KJ, Jiao K, Silver J, Deneris ES. Lmx1b is required at multiple stages to build expansive serotonergic axon architectures. Elife. 2019 PMID: 31355748

  3. Goiran T, Duplan E, Chami M, Bourgeois A, El Manaa W, Rouland L, Dunys J, Lauritzen I, You H, Stambolic V, Biféri MG, Barkats M, Pimplikar SW, Sergeant N, Colin M, Morais VA, Pardossi-Piquard R, Checler F, Alves da Costa C. β-Amyloid Precursor Protein Intracellular Domain Controls Mitochondrial Function by Modulating Phosphatase and Tensin Homolog-Induced Kinase 1 Transcription in Cells and in Alzheimer Mice Models. Biol Psychiatry. 2018 83(5):416-427. PMID: 28587718

  4. Shih DM, Zhu W, Schugar RC, Meng Y, Jia X, Miikeda A, Wang Z, Zieger M, Lee R, Graham M, Allayee H, Cantor RM, Mueller C, Brown JM, Hazen SL, Lusis AJ. Genetic Deficiency of Flavin-Containing Monooxygenase-3 ( Fmo3) Protects Against Thrombosis but Has Only a Minor Effect on Plasma Lipid Levels. Arterioscler Thromb Vasc Biol. 2019 PMID: 31070450

  5. Nandadasa S, Kraft CM, Wang LW, O'Donnell A, Patel R, Gee HY, Grobe K, Cox TC, Hildebrandt F, Apte SS. Secreted metalloproteases ADAMTS9 and ADAMTS20 have a non-canonical role in ciliary vesicle growth during ciliogenesis. Nat Commun. 2019 10(1):953. PMID: 30814516

  6. Shi Q, Ge Y, He W, Hu X, Yan R. RTN1 and RTN3 protein are differentially associated with senile plaques in Alzheimer's brains. Sci Rep. 2017 7(1):6145 PMID: 28733667

  7. Grandjean J, Corcoba A, Kahn MC, Upton AL, Deneris ES, Seifritz E, Helmchen F, Mann EO, Rudin M, Saab BJ. A brain-wide functional map of the serotonergic responses to acute stress and fluoxetine. Nat Commun. 2019 10(1):350. PMID: 30664643

  8. Lee H, Thacker S, Sarn N, Dutta R, Eng C. Constitutional mislocalization of Pten drives precocious maturation in oligodendrocytes and aberrant myelination in model of autism spectrum disorder. Transl Psychiatry. 2019 9(1):13. PMID: 30664625

  9. Baird RC, Li S, Wang H, Naga Prasad SV, Majdalany D, Perni U, Wu Q. Pregnancy-Associated Cardiac Hypertrophy in Corin-Deficient Mice: Observations in a Transgenic Model of Preeclampsia. Can J Cardiol. 2019 35(1):68-76. PMID: 30595185

2018
  1. Dudiki T, Joudeh N, Sinha N, Goswami S, Eisa A, Kline D, Vijayaraghavan S. The protein phosphatase isoform PP1γ1 substitutes for PP1γ2 to support spermatogenesis but not normal sperm function and fertility. Biol Reprod. 2019 100(3):721-736. PMID: 30379985

  2. Tanaka S, Suto A, Iwamoto T, Kageyama T, Tamachi T, Takatori H, Suzuki K, Hirose K, Ohara O, Lefebvre V, Nakajima H. Sox12 promotes T reg differentiation in the periphery during colitis. J Exp Med. 2018 Sep 6. PMID: 30190287

  3. Xiang X, Piers TM, Wefers B, Zhu K, Mallach A, Brunner B, Kleinberger G, Song W, Colonna M, Herms J, Wurst W, Pocock JM, Haass C. Mol Neurodegener. The Trem2 R47H Alzheimer's risk variant impairs splicing and reduces Trem2 mRNA and protein in mice but not in humans. 2018 13(1):49. PMID: 30185230

  4. Liu CF, Angelozzi M, Haseeb A, Lefebvre V. SOX9 is dispensable for the initiation of epigenetic remodeling and the activation of marker genes at the onset of chondrogenesis. Development. 2018 145(14). PMID: 30021842

  5. Zhu W, Buffa JA, Wang Z, Warrier M, Schugar R, Shih DM, Gupta N, Gregory JC, Org E, Fu X, Li L, DiDonato JA, Lusis AJ, Brown JM, Hazen SL. Flavin monooxygenase 3, the host hepatic enzyme in the metaorganismal trimethylamine N-oxide-generating pathway, modulates platelet responsiveness and thrombosis risk. J Thromb Haemost. 2018 PMID: 29981269

  6. McHugh DR, Steele MS, Valerio DM, Miron A, Mann RJ, LePage DF, Conlon RA, Cotton CU, Drumm ML, Hodges CA. A G542X cystic fibrosis mouse model for examining nonsense mutation directed therapies. PLoS One. 2018 13(6) PMID: 29924856
    • A disease-variant allele made by CRISPR/Cas9 mutagenesis.

  7. Cheng-Hathaway PJ, Reed-Geaghan EG, Jay TR, Casali BT, Bemiller SM, Puntambekar SS, von Saucken VE, Williams RY, Karlo JC, Moutinho M, Xu G, Ransohoff RM, Lamb BT, Landreth GE. The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease. Mol Neurodegener. 2018 13(1):29 PMID: 29859094
    • A disease-variant allele made by CRISPR/Cas9 mutagenesis.

  8. Meier ID, Walker MP, Matera AG. Gemin4 is an essential gene in mice, and its overexpression in human cells causes relocalization of the SMN complex to the nucleoplasm. Biol Open. 2018 PMID: 29371219

2017
  1. Orru CD, Yuan J, Appleby BS, Li B, Li Y, Winner D, Wang Z, Zhan YA, Rodgers M, Rarick J, Wyza RE, Joshi T, Wang GX, Cohen ML, Zhang S, Groveman BR, Petersen RB, Ironside JW, Quiñones-Mateu ME, Safar JG, Kong Q, Caughey B, Zou WQ. Prion seeding activity and infectivity in skin samples from patients with sporadic Creutzfeldt-Jakob disease. Sci Transl Med. 2017 9(417). PMID: 29167394

  2. Matarazzo V, Caccialupi L, Schaller F, Shvarev Y, Kourdougli N, Bertoni A, Menuet C, Voituron N, Deneris E, Gaspar P, Bezin L, Durbec P, Hilaire G, Muscatelli F. Necdin shapes serotonergic development and SERT activity modulating breathing in a mouse model for Prader-Willi Syndrome. Elife. 2017 PMID: 29087295

  3. Geng R, Omar A, Gopal SR, Chen DH, Stepanyan R, Basch ML, Dinculescu A, Furness DN, Saperstein D, Hauswirth W, Lustig LR, Alagramam KN. Modeling and Preventing Progressive Hearing Loss in Usher Syndrome III. Sci Rep. 2017 7(1):13480. PMID: 29044151

  4. Vithayathil J, Pucilowska J, Friel D, Landreth GE. Chronic impairment of ERK signaling in glutamatergic neurons of the forebrain does not affect spatial memory retention and LTP in the same manner as acute blockade of the ERK pathway. Hippocampus. 2017 PMID: 28833860

  5. Wichman L, Somasundaram S, Breindel C, Valerio DM, McCarrey JR, Hodges CA, Khalil AM. Dynamic expression of long non-coding RNAs reveals their potential roles in spermatogenesis and fertility. Biol Reprod 2017
    • This publication describes an lncRNA gene deletion generated with CRISPR/Cas9 by the core

  6. Hutton SR, Otis JM, Kim EM, Lamsal Y, Stuber GD, Snider WD. ERK/MAPK signaling is required for pathway-specific striatal motor functions. J Neurosci. 2017

  7. Soler DC, Young AE, Griffith AD, Fu PF, Cooper KD, McCormick TS, Popkin DL. Overexpression of AQP3 and AQP10 in the skin exacerbates psoriasiform acanthosis. Exp Dermatol. 2017 PMID: 28111811

  8. Seachrist DD, Sizemore ST, Johnson E, Abdul-Karim FW, Weber Bonk KL, Keri RA. Follistatin is a metastasis suppressor in a mouse model of HER2-positive breast cancer. Breast Cancer Res. 2017 PMID: 28583174

  9. Nguyen HT, Hinman MN, Guo X, Sharma A, Arakawa H, Luo G, Lou H. Neurofibromatosis type 1 alternative splicing is a key regulator of Ras/ERK signaling and learning behaviors in mice. Hum Mol Genet 2017

  10. Wilk K, Yeh SA, Mortensen LJ, Ghaffarigarakani S, Lombardo CM, Bassir SH, Aldawood ZA, Lin CP, Intini G. Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration. Stem Cell Reports. 2017 PMID: 28366454

  11. Kurtsdotter I, Topcic D, Karlén A, Singla B, Hagey DW, Bergsland M, Siesjö P, Nistér M, Carlson JW, Lefebvre V, Persson O, Holmberg J, Muhr J. SOX5/6/21 prevent oncogene-driven transformation of brain stem cells. Cancer Res. 2017 PMID: 28687615

  12. Schugar RC, Shih DM, Warrier M, Helsley RN, Burrows A, Ferguson D, Brown AL, Gromovsky AD, Heine M, Chatterjee A, Li L, Li XS, Wang Z, Willard B, Meng Y, Kim H, Che N, Pan C, Lee RG, Crooke RM, Graham MJ, Morton RE, Langefeld CD, Das SK, Rudel LL, Zein N, McCullough AJ, Dasarathy S, Tang WHW, Erokwu BO, Flask CA, Laakso M, Civelek M, Naga Prasad SV, Heeren J, Lusis AJ, Hazen SL, Brown JM. The TMAO-Producing Enzyme Flavin-Containing Monooxygenase 3 Regulates Obesity and the Beiging of White Adipose Tissue. Cell Rep. 2017 19(12):2451-2461. PMID: 28636934
    • This publication describes Fmo3 knockout mice generated with CRISPR/Cas9 by the core

  13. Khoriaty R, Vogel N, Hoenerhoff MJ, Sans MD, Zhu G, Everett L, Nelson B, Durairaj H, McKnight B, Zhang B, Ernst SA, Ginsburg D, Williams JA. SEC23B is required for pancreatic acinar cell function in adult mice. Mol Biol Cell. 2017 PMID: 28539403

  14. Darrah R, Bederman I, Vitko M, Valerio DM, Drumm ML, Hodges CA. Growth deficits in cystic fibrosis mice begin in utero prior to IGF-1 reduction. PLoS One. 2017 12(4):e0175467. PMID: 28384265

  15. Mamidi R, Gresham KS, Li J, Stelzer JE. Cardiac myosin binding protein-C Ser302 phosphorylation regulates cardiac β-adrenergic reserve. Sci Adv. 2017 3(3):e1602445. PMID: 28345052

  16. Kuwajima T, Soares CA, Sitko AA, Lefebvre V, Mason C. SoxC Transcription Factors Promote Contralateral Retinal Ganglion Cell Differentiation and Axon Guidance in the Mouse Visual System. Neuron. 2017 PMID: 28215559

All Publications